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EntroGen Inc
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EntroGen Inc
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BML Inc
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OSI Pharmaceuticals
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EntroGen Inc
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Nagai Nori USA INC
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Johns Hopkins HealthCare
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Devyser AB
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Response Genetics Inc
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LabCorp
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Image Search Results
Journal: Molecular Diagnosis & Therapy
Article Title: Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics
doi: 10.1007/s40291-022-00612-3
Figure Lengend Snippet: Examples of the most common pathogenic variants detected by NGS. The black arrows show the position of the detected variant. Abnormalities were assessed using TIER 2017 Variant Classification and AMCG 2015. A TERT promoter upstream gene variant NC_000005.9: g.1295228G>A TIER I; pathogenic. B TERT promoter upstream gene variant NC_000005.9:g.1295250G>A TIER I; pathogenic. C IDH1 R132H missense variant NM_005896.2:c.395G>A (p.Arg132His) TIER I. D IDH1 R132S missense variant NM_005896.2:c.394C>A (p.Arg132Ser) TIER II; Likely pathogenic. E PIK3CA missense variant NM_006218.2:c.1633G>A (p.Glu545Lys) TIER I; pathogenic. F BRAF missense variant NM_004333.4:c.1799T>A (p.Val600Glu) TIER I; pathogenic. NGS next-generation sequencing
Article Snippet: The black arrows indicate mutations detected using next-generation sequencing (NGS Targeted Hotspot Panel, Entrogen), Sanger sequencing (Applied Biosystems SeqStudio Genetic Analyzer), and
Techniques: Variant Assay, Next-Generation Sequencing
Journal: Molecular Diagnosis & Therapy
Article Title: Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics
doi: 10.1007/s40291-022-00612-3
Figure Lengend Snippet: Evaluation of IDH1 status obtained by non-reference center (IHC), with 3 molecular methods (NGS, qPCR, Sanger) obtained by reference oncology center
Article Snippet: The black arrows indicate mutations detected using next-generation sequencing (NGS Targeted Hotspot Panel, Entrogen), Sanger sequencing (Applied Biosystems SeqStudio Genetic Analyzer), and
Techniques: Mutagenesis
Journal: Molecular Diagnosis & Therapy
Article Title: Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics
doi: 10.1007/s40291-022-00612-3
Figure Lengend Snippet: Evaluation of IDH1 status received from non-reference center (MLPA), with 3 molecular methods (NGS, qPCR, Sanger) received from reference oncology center
Article Snippet: The black arrows indicate mutations detected using next-generation sequencing (NGS Targeted Hotspot Panel, Entrogen), Sanger sequencing (Applied Biosystems SeqStudio Genetic Analyzer), and
Techniques: Mutagenesis
Journal: Molecular Diagnosis & Therapy
Article Title: Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics
doi: 10.1007/s40291-022-00612-3
Figure Lengend Snippet: Examples of IHC and MLPA reports from different non-oncological reference centers versus NGS, qPCR, and Sanger sequencing results from the oncology reference center. Two false positives were detected by MLPA and IHC, and one false negative by IHC. Pathogenic variants detected by NGS were additionally validated by two independent methods: Sanger sequencing and qPCR. The black arrows indicate mutations detected using next-generation sequencing (NGS Targeted Hotspot Panel, Entrogen), Sanger sequencing (Applied Biosystems SeqStudio Genetic Analyzer), and qPCR ( IDH1/2 Mutation Detection Kit, Entrogen), with a limit of detection of 5, 20, and 1%, respectively. Two red curves represent (1) positive control and (2) amplicon with mutation detected ( arrow ). The patient no. 13 – MLPA report indicates IDH-wildtype, while NGS, qPCR, and Sanger sequencing confirmed mutation in the IDH1 gene. IHC immunohistochemistry, MLPA multiplex ligation-dependent probe amplification, NGS next-generation sequencing, qPCR quantitative polymerase chain reaction
Article Snippet: The black arrows indicate mutations detected using next-generation sequencing (NGS Targeted Hotspot Panel, Entrogen), Sanger sequencing (Applied Biosystems SeqStudio Genetic Analyzer), and
Techniques: Sequencing, Next-Generation Sequencing, Mutagenesis, Positive Control, Amplification, Immunohistochemistry, Multiplex Assay, Ligation, Real-time Polymerase Chain Reaction
Journal: Molecular Diagnosis & Therapy
Article Title: Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics
doi: 10.1007/s40291-022-00612-3
Figure Lengend Snippet:
Article Snippet: The black arrows indicate mutations detected using next-generation sequencing (NGS Targeted Hotspot Panel, Entrogen), Sanger sequencing (Applied Biosystems SeqStudio Genetic Analyzer), and
Techniques: Next-Generation Sequencing, Real-time Polymerase Chain Reaction
Journal: Journal of Clinical Oncology
Article Title: A Randomized, Phase II, Biomarker-Selected Study Comparing Erlotinib to Erlotinib Intercalated With Chemotherapy in First-Line Therapy for Advanced Non-Small-Cell Lung Cancer
doi: 10.1200/JCO.2010.34.4929
Figure Lengend Snippet: Biomarker Results On the Basis of Treatment Arm and Key Patient Characteristics
Article Snippet: With consent, the remnant tissue was used for EGFR mutation testing by Genzyme Genetics (Westborough, MA) and
Techniques: Biomarker Assay, Mutagenesis
Journal: Journal of Clinical Oncology
Article Title: A Randomized, Phase II, Biomarker-Selected Study Comparing Erlotinib to Erlotinib Intercalated With Chemotherapy in First-Line Therapy for Advanced Non-Small-Cell Lung Cancer
doi: 10.1200/JCO.2010.34.4929
Figure Lengend Snippet: Thirty-six patients were fluorescent in situ hybridization negative, KRAS wild type (WT), and EGFR WT.
Article Snippet: With consent, the remnant tissue was used for EGFR mutation testing by Genzyme Genetics (Westborough, MA) and
Techniques: In Situ Hybridization
Journal: Journal of Clinical Oncology
Article Title: A Randomized, Phase II, Biomarker-Selected Study Comparing Erlotinib to Erlotinib Intercalated With Chemotherapy in First-Line Therapy for Advanced Non-Small-Cell Lung Cancer
doi: 10.1200/JCO.2010.34.4929
Figure Lengend Snippet: Analyses of PFS and OS for Biomarker Subsets
Article Snippet: With consent, the remnant tissue was used for EGFR mutation testing by Genzyme Genetics (Westborough, MA) and
Techniques: Biomarker Assay, Mutagenesis
Journal: Journal of Clinical Oncology
Article Title: A Randomized, Phase II, Biomarker-Selected Study Comparing Erlotinib to Erlotinib Intercalated With Chemotherapy in First-Line Therapy for Advanced Non-Small-Cell Lung Cancer
doi: 10.1200/JCO.2010.34.4929
Figure Lengend Snippet: Response by Biomarker Status
Article Snippet: With consent, the remnant tissue was used for EGFR mutation testing by Genzyme Genetics (Westborough, MA) and
Techniques: Biomarker Assay, Mutagenesis